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PRODUCT INFORMATION:
Form
Description
Human Angiostatin K1-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PLG in cell culture supernatants, serum and plasma (heparin, EDTA).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Host
Contents
Clonality
Clone Number
Isotype
Immunogen
Expression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
Human
Applications
ELISA
Background
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED =70 nM) relative to kringles 1-4 (ED = 135 nM).
ASSAY DILUTION:
Reconstitution
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
PROTEIN TARGET INFO:
Uniprot ID
P00747
Gene Name
PLG
Full Name
plasminogen
Weight
Superfamily
actin family
Alternative Names
Plasminogen; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG
Datasheet MSDS
