Description
Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.
Features
- Performs under a wide range of reaction conditions
- Protects RNA from degradation at temperatures up to 55°C
Specifications
| Concentration | 40 U/μL |
| Product Type | RNase Inhibitor |
| Unit Definition | The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2′, 3′-CMP to generate 3′-CMP. |
Components
| Components No. | Name | 10603ES05 (2 KU) |
| 10603 | Murine RNase Inhibitor (40 U/µL) | 50 μL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -20°C for 2 years.
Figure
Figure 1. RNase inhibitor can effectively inhibit RNase A activity to prevent the digestion of total RNA from HEK 293 cells.
