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Tissue homogenization is a crucial step for RNA extraction, as it allows the release of RNA molecules from the cells and tissues. However, tissue homogenization also poses some challenges, such as the risk of RNA degradation, contamination, and loss. Therefore, it is important to follow some best practices to ensure optimal tissue homogenization for RNA …

What Are the Considerations on Tissue Homogenization for RNA Extraction? Read More »

Cryogenic preservation is a technique that allows cells to be stored at very low temperatures, usually in liquid nitrogen, for long periods of time without losing their viability or functionality. Cryogenic preservation is useful for preserving valuable cell lines, avoiding genetic drift or contamination, and reducing the cost and labor of maintaining cells in culture. …

What Are the Considerations on Reviving Cells from Cryogenic Preservation? Read More »

Exosomes are small membrane-bound vesicles that are released by various types of cells and carry a variety of molecules, such as proteins, lipids, and nucleic acids. Exosomes play important roles in intercellular communication, immune regulation, disease progression, and biomarker discovery. Therefore, isolating and characterizing exosomes from biological fluids or cell culture supernatants is a valuable …

What Are the Methods to Isolate Exosomes – A Comprehensive Overview Read More »

Cell culture is a technique that involves growing cells in an artificial environment outside their natural tissue or organ. Cell culture is widely used in biomedical research, drug development, tissue engineering, and regenerative medicine. However, not all cell culture methods are the same. Depending on how the cells are arranged and interact with their surroundings, …

2D vs 3D Cell Culture: What Are the Differences? Read More »

Maintaining cultured cells is a crucial skill for any life science researcher who works with cell-based assays or experiments. Cultured cells are living organisms that require optimal conditions and care to grow and function properly. In this blog post, I will outline some of the key aspects and tips for maintaining cultured cells. Types of …

How to Maintain Cultured Cells: A Basic Overview Read More »

Cryopreservation is a method of preserving cells by freezing them at very low temperatures, usually in liquid nitrogen. Cryopreservation allows cells to be stored for long periods of time without losing their viability or functionality. Cryopreservation is useful for maintaining cell lines, avoiding contamination, minimizing genetic drift, and saving space and resources. In this blog …

How to Cryopreserve Mammalian Cell Lines? Read More »

Cell adhesion is the process by which cells attach to each other or to a substrate, such as a culture surface. Cell adhesion is essential for many biological functions, such as tissue formation, wound healing, immune response, and cancer metastasis. In mammalian cell culture, cell adhesion can affect the growth, differentiation, and survival of the …

What Are the Factors That Affect Cell Adhesion in Mammalian Cell Culture? Read More »

DNA, or deoxyribonucleic acid, is the molecule that carries the genetic information of living organisms. DNA can be extracted from various sources, such as blood, saliva, tissue, cells, etc. However, before using DNA for further analysis or applications, such as PCR, sequencing, cloning, or gene editing, it is important to know how much DNA you …

What Are the Methods to Quantify DNA? Read More »

PCR, or polymerase chain reaction, is a technique that allows you to amplify a specific segment of DNA or RNA from a sample. PCR can be used for various purposes, such as diagnosing diseases, identifying pathogens, detecting mutations, analyzing gene expression, and more. But what is the annealing temperature in PCR and how do you …

How to Calculate the Optimal Annealing Temperature for PCR Reaction Read More »

PCR, or polymerase chain reaction, is a technique that allows you to amplify a specific segment of DNA or RNA from a sample. PCR can be used for various purposes, such as diagnosing diseases, identifying pathogens, detecting mutations, analyzing gene expression, and more. But what do you need to run a PCR test in your …

Supplies and Equipment to Run a PCR Test: A Basic Overview Read More »